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14 i I -7 -1 I 0 i 1 , , 2 3 I I 7 I 14 (Day) Clip each ~ animal free of hair on back and sides ~Single Closed Patch Application to Skin Site for 4 Hours *Sites observed and evaluated at 4 and 48 hours FIGURE 7 Line chart for design and conduct of dermal corrosivity study in the rabbits. *Sites observed and evaluated at 4 and 48 hours. A. Rabbit screening procedure 1. A group of at least 8-12 New Zealand White rabbits is screened for the study. 2. All rabbits selected for the study must be in good health; any rabbit exhibiting snuffles, hair loss, loose stools, or apparent weight loss is rejected and replaced.
And Kunstler, K. (1989). Skin organ culture for the study of skin irritancy. Third International Congress of Toxicology, Brighton, UK. , et al. (1989). Use of fabricated living tissue and organ equivalents as defined in higher order systems for the study of pharmacologic responses to test substances. Presented at the NATO Advanced Research Workshop, Pharmaceutical Application of Cell and Tissue Culture to Drug Transport, Bandol, France, September 4-9. Boyce, S. , Hansbrough, J. , and Norris, D.
5 ml of physiological saline. ). The positive control substance and vehicle control substance are applied to a gauze patch in the same manner as a liquid test substance. The entire trunk of the animal is covered with an impervious material (such as Saran Wrap) for a 24-hr period of exposure. The Saran Wrap is secured by wrapping several long strips of athletic adhesive tape around the trunk of the animal. The impervious material aids in maintaining the position of the patches and retards evaporation of volatile test substances.